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Updated: Apr 11, 2026
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
Published on: February 17, 2017
A terthiophene-derived fluorescent probe with long-emission wavelength for highly sensitive detection of heparin and
Gongli Wei1, Ying Feng1, Li Zhao1
1School of Light Industry, Beijing Technology and Business University, Beijing 102488, China.
Abstract:
The determination of heparin and protamine is of significant importance in clinical medicine. An effective fluorescent sensing strategy was designed based on a long-wavelength cationic probe. The 4-methyl-1-(3-(trimethylammonio)propyl)pyridin-1-ium bromide (TAPP) moiety was introduced into terthiophene (3 T), yielding a cationic fluorescent probe (3T-TAPP) with red emission. The red emission was quenched due to H-type aggregation of 3T-TAPP, resulting from electrostatic binding between 3T-TAPP and heparin. The sensitivity and selectivity of the probe for heparin were satisfactory in both HEPES buffer and 10% serum. Furthermore, the 3T-TAPP/heparin complex exhibited sensing capability for protamine. 3T-TAPP was released from the 3T-TAPP/heparin complex because protamine has a stronger binding affinity to heparin than 3T-TAPP. The sensitivity and selectivity of the assay for protamine were confirmed in both HEPES buffer and 10% serum. This work proposes a strategy for constructing red-emission probes for the detection of biomolecules.
