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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

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An isaindigotone-based NIR fluorogenic probe for visualizing mitochondrial DNA G-quadruplexes in living cells.

Zhaocheng Liu1, Yinxia Liu2, Xiaoqing Wang1

  • 1College of Chemistry and Material Science, Hengyang Normal University, Hengyang 421001, PR China.

Chemical Communications (Cambridge, England)
|April 13, 2026
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Summary

Researchers developed a new fluorescent probe, IS-HX, to visualize mitochondrial DNA G-quadruplexes (mtDNA G4s) in living cells. This probe allows for the dynamic monitoring of mtDNA G4s, offering insights into mitochondrial function.

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Area of Science:

  • Mitochondrial biology
  • Molecular imaging
  • Biochemistry

Background:

  • Mitochondrial DNA G-quadruplexes (mtDNA G4s) are increasingly recognized for their role in regulating mitochondrial function.
  • Specific tools are needed to visualize and study these structures in real-time within living cells.

Purpose of the Study:

  • To design and characterize a novel fluorescent probe for the specific detection and imaging of mtDNA G4s in living cells.
  • To utilize the probe to monitor dynamic changes in mtDNA G4s under specific cellular conditions.

Main Methods:

  • Design and synthesis of a novel fluorescent probe (IS-HX) based on a fluorinated isaindigotone scaffold and dihydroxanthene units.
  • Characterization of the probe's spectral properties (near-infrared absorption and emission) and its selectivity/specificity for mtDNA G4s.
  • Application of the probe for live-cell imaging to observe dynamic changes in mtDNA G4s.

Main Results:

  • The IS-HX probe demonstrated near-infrared (NIR) optical properties.
  • IS-HX exhibited high selectivity and specificity for imaging mtDNA G4s.
  • The probe successfully visualized dynamic changes of mtDNA G4s in cells under glycolysis-induced conditions.

Conclusions:

  • The novel IS-HX fluorescent probe is effective for specific and selective imaging of mtDNA G4s in living cells.
  • IS-HX provides a valuable tool for studying the dynamic behavior of mtDNA G4s and their role in cellular processes.
  • This probe facilitates research into mitochondrial function and related pathologies.