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Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

15.1K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Updated: Apr 14, 2026

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
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Cellular Characterization Using Microwave-Assisted Immunocytochemistry, A Time-Efficient Alternative to Traditional

Aidyn M Medina-López1,2, Irma I Torres-Vázquez3, Noraida Martínez-Rivera4

  • 1Ronawk, Inc. Overland Park, KS, USA.

Current Protocols
|April 13, 2026
PubMed
Summary

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are distinct techniques for cell and tissue analysis. Assisted ICC offers a rapid, reproducible method for characterizing cell lines by reducing background noise without affecting protein expression.

Keywords:
blood‐spinal cord barrier (BSCB)cell characterizationimmunocytochemistrymicrowave‐assisted stainingneurovascular unit

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Area of Science:

  • Cell biology
  • Biochemistry
  • Immunology

Background:

  • Immunocytochemistry (ICC) and immunohistochemistry (IHC) are crucial for characterizing cells and tissues.
  • The distinction between ICC and IHC, primarily based on sample thickness, can be unclear.
  • Clear differentiation is essential for accurate scientific communication and experimental design.

Purpose of the Study:

  • To clarify the distinction between ICC and IHC.
  • To present assisted ICC as an effective method for cell line characterization.
  • To highlight the benefits of assisted ICC for improving experimental rigor and reproducibility.

Main Methods:

  • Comparison of immunocytochemistry (ICC) and immunohistochemistry (IHC) principles.
  • Development and application of assisted ICC using PELCO BioWave® Pro.
  • Culture of blood-spinal cord barrier and neurovascular unit cellular components.

Main Results:

  • Assisted ICC effectively characterizes cell lines.
  • This method is rapid and reduces background noise.
  • Protein expression and organization remain undisturbed, enhancing result reliability.

Conclusions:

  • A clear distinction between ICC and IHC is necessary.
  • Assisted ICC is a valuable technique for cell line characterization.
  • Assisted ICC improves the rigor and reproducibility of scientific findings.