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Related Experiment Video

Updated: Apr 15, 2026

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

Ultra-wide-field, deep, adaptive two-photon microscopy for multi-scale neuronal imaging.

Mengke Yang^1,2, Zhen-Qiao Zhou^1,3,4, Song Lang^1,3,4

¹Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.

Light, Science & Applications

|April 13, 2026

Summary

Researchers developed a new ultra-large field of view two-photon microscopy system for in vivo brain imaging. This advanced technology enables high-resolution visualization of neural activity across wide areas and depths, overcoming current limitations.

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Last Updated: Apr 15, 2026

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

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Published on: December 12, 2012

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Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

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Published on: January 19, 2024

Area of Science:

Neuroscience
Biomedical Imaging
Optical Microscopy

Background:

Understanding brain function requires observing large neural populations.
Existing microscopy methods struggle to image neural interactions across distributed regions and cortical layers.

Purpose of the Study:

Introduce a novel two-photon microscopy system, ULTRA.
Enable deep and wide-field in vivo imaging with single-cell resolution across an ultra-large field of view.

Main Methods:

Developed ULTRA, a two-photon microscopy system.
Achieved an ultra-large field of view (>50 mm²).
Performed in vivo imaging in the mouse brain.

Main Results:

Successfully visualized brain structures and neuronal activities across a 7 mm spatial range.
Imaged from superficial layers to depths of 900 μm.
Covered an imaging volume of 45.24 mm³.

Conclusions:

ULTRA overcomes traditional spatial limitations in brain imaging.
Provides a versatile platform for comprehensive neuronal circuitry exploration at extensive spatial scales.
Enables cellular-resolution imaging across wide areas and depths.