Multiomics Analysis Reveals the Protective Effect of a Novel Bioactive Peptide (BP1) in Cardiomyopathy Using a...

Area of Science:

• Cardiovascular Research
• Pharmacology
• Zebrafish Models

Background:

• Medicinal plants are sources of bioactive peptides (BPs) with therapeutic potential.
• Bioactive peptide BP1 was isolated from Picrorhiza kurroa.
• BP1's cardioprotective role and metabolic control require system-level understanding.

Purpose of the Study:

• Investigate the cardioprotective effect of BP1.
• Assess BP1's efficacy in zebrafish models of isoproterenol- and doxorubicin-induced cardiac damage.
• Elucidate the molecular and metabolic mechanisms underlying BP1's cardioprotection.

Main Methods:

• Utilized zebrafish larvae and adult models for isoproterenol (ISO) and doxorubicin (DOX) induced cardiotoxicity.
• Evaluated cardiac morphology and function post-BP1 treatment.
• Performed transcriptomic and metabolomic analyses, including WGCNA.

Main Results:

• BP1 significantly reduced ISO-induced cardiac dysfunction in zebrafish larvae.
• BP1 pretreatment mitigated DOX-induced myocardial pathological changes.
• Transcriptomic and metabolomic data revealed BP1 targets protein synthesis, metabolic pathways, and cardiac contraction, attenuating cardiotoxicity.

Conclusions:

• BP1 exhibits significant cardioprotective effects in zebrafish models.
• BP1 acts via targeting multiple pathogenic pathways involved in cardiotoxicity.
• BP1 represents a promising therapeutic agent for cardiovascular protection.