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Miniaturized chromatography-based lipidomics methods towards single-cell analysis.

Klidel Fae Rellin1,2, Michael Witting3,4

  • 1Metabolomics and Proteomics Core, Helmholtz Zentrum München German Research Center for Environmental Health, 85764, Neuherberg, Germany.

Analytical and Bioanalytical Chemistry
|April 20, 2026
PubMed
Summary
This summary is machine-generated.

Miniaturized chromatography enhances lipidomics by enabling analysis of tiny samples, revealing cellular details. This technology is key for single-cell lipid studies and understanding metabolism.

Keywords:
Ion mobilityLipidomicsLiquid chromatographyMass spectrometryNanoLCSingle cell lipidomics

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Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Conventional lipidomics using bulk samples obscures cellular lipid heterogeneity.
  • Advances in analytical chemistry allow lipid analysis from minute biological samples.

Purpose of the Study:

  • To review recent advancements in miniaturized chromatography for lipidomics.
  • To highlight technologies enabling low-input and single-cell lipid analysis.
  • To discuss challenges and future directions in the field.

Main Methods:

  • Focuses on miniaturized liquid chromatography-mass spectrometry (LC-MS) workflows.
  • Discusses low-flow electrospray interfaces and ion mobility (IM) spectrometry.
  • Addresses sample preparation and quantification for picogram-scale analytes.

Main Results:

  • Miniaturization improves ionization efficiency, sensitivity, and separation performance.
  • Reduced sample consumption is achieved through scaled-down chromatography.
  • Ion mobility spectrometry provides an orthogonal separation dimension for enhanced lipid identification.

Conclusions:

  • Miniaturized chromatography is crucial for advancing single-cell lipidomics.
  • Developments in automation, microfluidics, and multidimensional separations are promising.
  • This platform facilitates high-resolution studies of lipid metabolism and cellular function.