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Noninvasive Skin Surface Sampling Identifies Elevated IL-1β and IL-6 in Paediatric Atopic Dermatitis Patients.
Amir Horev1, Sapir Gabay2, Yuliya Valdman-Grinshpoun3
1Pediatric Dermatology Service, Soroka University Medical Center, Beer-Sheva, Israel; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Acta Dermato-Venereologica
|April 23, 2026
Summary
This study shows non-invasive skin surface sampling can detect elevated inflammatory markers like interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) in children with atopic dermatitis (AD), aiding biomarker discovery.
Area of Science:
- Dermatology
- Immunology
- Pediatrics
Background:
- Atopic dermatitis (AD) molecular signatures are not fully understood.
- Previous studies on AD inflammation markers used invasive methods like biopsies.
- Non-invasive skin surface sampling in pediatric AD is underexplored.
Purpose of the Study:
- To identify and compare cytokine expression on the skin surface of children with AD and healthy controls.
- To assess the feasibility of non-invasive cytokine sampling in pediatric AD.
- To explore factors influencing cytokine expression on the skin surface.
Main Methods:
- Recruited 50 children (40 with mild-to-moderate AD, 10 healthy controls).
- Collected skin surface samples using sterile phosphate-buffered saline-soaked sticky bandages.
- Measured 15 cytokine concentrations via multiplex immunoassay; analyzed data using redundancy analysis (RDA) and factorial ANOVA.
Main Results:
- Elevated IL-1β levels were found in non-lesional skin of AD patients compared to controls.
- Increased IL-1β and IL-6 expression was observed in lesional AD skin versus non-lesional skin.
- Skin folds and sex were identified as factors influencing cytokine secretion.
Conclusions:
- Non-invasive cytokine sampling from the skin surface is feasible in pediatric AD.
- This method can identify inflammatory biomarkers, complementing studies on deeper skin layers.
- Findings highlight potential for new diagnostic and monitoring tools in pediatric AD.
