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Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
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Total Nitrogen Determination by a Spectrophotometric Method.

Jaana Koistinen1, Mervi Sjöblom2, Kristian Spilling3,4

  • 1Tvärminne Zoological Station, University of Helsinki, Hanko, Finland. jaana.koistinen@helsinki.fi.

Methods in Molecular Biology (Clifton, N.J.)
|April 30, 2026
PubMed
Summary

Measuring total nitrogen (TN) is crucial for nitrogen budget tracking. This chapter details a spectrophotometric method using persulfate digestion and nitrate determination for accurate TN analysis.

Keywords:
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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry

Background:

  • Accurate monitoring of total nitrogen (TN) is essential for understanding and managing nitrogen budgets in various environmental and biological systems.
  • Traditional methods for TN determination can be complex or time-consuming, necessitating efficient and reliable analytical techniques.

Purpose of the Study:

  • To present a robust spectrophotometric method for the precise determination of total nitrogen (TN).
  • To outline the key steps involved in the oxidation and reduction processes for TN analysis.

Main Methods:

  • The described method involves a persulfate digestion step to convert all nitrogenous compounds into nitrate.
  • Subsequent spectrophotometric analysis is performed to quantify the nitrate concentration, which directly correlates to the total nitrogen content.

Main Results:

  • The spectrophotometric method provides a reliable means for measuring total nitrogen.
  • The process ensures comprehensive conversion of nitrogen compounds, enabling accurate TN quantification.

Conclusions:

  • The presented spectrophotometric method offers an effective approach for total nitrogen (TN) measurement.
  • This technique is valuable for environmental monitoring and research requiring precise nitrogen budget assessments.