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Ionic Extracts of Magnesium Powders Promote In Vitro Lymphangiogenesis
Yan Wang1,2,3, Xiran Liu2, Zerui Shan1,2,3
1State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing Medical University, Nanjing 210029, China.
Biomedicines
|May 4, 2026
Summary
Magnesium-based biomaterials promote lymphatic endothelial cell growth and tube formation in vitro. These findings suggest potential for magnesium in lymphatic tissue regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Vascular Biology
Background:
- Biodegradable magnesium (Mg)-based biomaterials release Mg2+ ions, potentially aiding vascular regeneration.
- The impact of Mg2+ on lymphatic endothelial cells (LECs) and lymphangiogenesis is not well understood.
Purpose of the Study:
- To investigate if magnesium powder-derived ionic extracts enhance lymphangiogenesis-related behaviors of LECs in vitro.
- To assess the effects of Mg extracts on LEC proliferation, migration, and tube formation.
Main Methods:
- Mg powder extracts were prepared and diluted (1:10, 1:100, 1:1000) for in vitro LEC treatment.
- LEC proliferation, migration, and tube formation assays were performed.
- Intracellular reactive oxygen species (ROS) levels and expression of VEGFA, VEGFC, and VEGFR3 were measured.
Main Results:
- Mg extracts at 1:10 and 1:100 dilutions demonstrated good cytocompatibility.
- These concentrations significantly promoted LEC proliferation, migration, and tube formation compared to controls.
- Reduced intracellular ROS levels and increased VEGFA, VEGFC, and VEGFR3 expression were observed.
Conclusions:
- Magnesium powder-derived ionic extracts enhance lymphangiogenesis-related responses in LECs.
- The 1:10 and 1:100 dilutions show particular promise for promoting lymphatic regeneration.
- Mg-based biomaterials hold potential for applications in lymphatic tissue repair.
