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Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Related Experiment Video

Updated: May 5, 2026

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

Published on: December 30, 2012

Efficient and Stable Subcellular Protein Labeling in Leishmania mexicana Using a Re-Engineered mNeonGreen Integration

Tianyu Lei¹, Mengtao Yu¹, Panjing Lv¹

¹Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Huazhong University of Science and Technology, Wuhan 430030, China.

Pathogens (Basel, Switzerland)

|May 4, 2026

Summary

This summary is machine-generated.

Researchers developed a new molecular toolbox for Leishmania mexicana, using a bright mNeonGreen fluorophore for stable protein localization. This tool enhances high-resolution imaging of parasite organelle dynamics.

Keywords:

Leishmania mexicana bioimaging flagellum mNeonGreen protein localization

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Last Updated: May 5, 2026

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

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Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production

Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production

Published on: November 8, 2024

Area of Science:

Parasitology
Molecular Biology
Cell Biology

Background:

Leishmania mexicana is a key model for trypanosomatid research.
Existing fluorescent tools lack stability and intensity for precise subcellular localization.

Purpose of the Study:

To develop a stable, high-intensity fluorescent reporter system for Leishmania mexicana.
To enable precise subcellular protein localization and high-resolution imaging.

Main Methods:

Re-engineered the pLEXSY-hyg2.1 vector to express mNeonGreen (mNG).
Utilized modular cloning for N-terminal protein tagging.
Integrated the construct into the 18S rRNA locus for stable expression.

Main Results:

Created a versatile molecular toolbox with a bright, photostable mNG fluorophore.
Demonstrated stable, constitutive expression of tagged proteins.
Generated a proof-of-concept transgenic line with specific flagellar localization.

Conclusions:

The integration-based system provides an efficient and stable platform for Leishmania protein visualization.
This tool simplifies reporter strain generation for high-resolution imaging and functional genomics.