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Spontaneous calcium release from sarcoplasmic reticulum. A re-examination
Biochimica Et Biophysica Acta
|March 8, 1979
Summary
Spontaneous calcium release from sarcoplasmic reticulum is not an artifact of murexide dye uptake. This calcium release phenomenon can be reversed by ATP or oxalate and confirmed with other methods.
Area of Science:
- Biochemistry
- Cell Biology
- Muscle Physiology
Background:
- A previous study suggested spontaneous calcium release from sarcoplasmic reticulum (SR) was an artifact of murexide dye.
- This artifact was proposed to be due to murexide dye uptake during calcium release measurements.
Purpose of the Study:
- To investigate whether spontaneous calcium release from sarcoplasmic reticulum (SR) is an artifact of murexide dye.
- To validate the phenomenon of spontaneous calcium release using alternative methods.
Main Methods:
- Equilibration of murexide with SR vesicles to a stable baseline.
- Monitoring calcium release using murexide, arsenazo III, or Millipore filtration.
- Addition of ATP or oxalate to observe effects on calcium release.
Main Results:
- Spontaneous calcium release occurred even after murexide-SR equilibration to a stable baseline.
- Calcium release was reversed upon addition of ATP or oxalate.
- The phenomenon was reproducible using arsenazo III and Millipore filtration methods.
Conclusions:
- Spontaneous calcium release from sarcoplasmic reticulum is a genuine phenomenon.
- Proper equilibration of murexide with SR vesicles is crucial for a stable baseline.
- The findings challenge the artifact hypothesis and support the reality of spontaneous calcium release.