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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

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Related Experiment Video

Updated: May 8, 2026

Generation of Alginate Microspheres for Biomedical Applications
10:33

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Published on: August 12, 2012

Injectable, electrosprayed RGD-coupled alginate hydrogel microcapsules enable enhanced viability and sustained

Dominic Karl M Bolinas1,2, Allan John R Barcena1, Archana Mishra1

  • 1Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Unit 1471, 1515 Holcombe Blvd, Houston, TX, 77030, USA.

Biomedical Microdevices
|May 7, 2026
PubMed
Summary
This summary is machine-generated.

Electrosprayed arginine-glycine-aspartic acid (RGD)-coupled alginate microcapsules enhance mesenchymal stem cell (MSC) viability and enable sustained release for regenerative medicine.

Keywords:
ElectrosprayHydrogelsMesenchymal stem cellsRGD-Alginate

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cell Therapy Engineering

Background:

  • Clinical translation of mesenchymal stem cell (MSC) therapies is hindered by rapid cell clearance and stress-induced viability loss.
  • Alginate (Alg) microencapsulation is a promising strategy, with arginine-glycine-aspartic acid (RGD)-coupled alginate (RAlg) showing improved bioactivity.
  • Challenges remain in achieving precise encapsulation, injectability, and sustained cell viability.

Purpose of the Study:

  • To develop an injectable delivery platform using electrosprayed RAlg microcapsules for enhanced MSC viability and sustained release.
  • To optimize electrospray parameters for uniform microcapsule production.
  • To evaluate the physicochemical properties, cell viability, and release kinetics of MSCs encapsulated in RAlg microcapsules.

Main Methods:

  • Electrospray technique was employed to fabricate RAlg microcapsules.
  • Microcapsule size, morphology, and internal architecture were characterized using microscopy.
  • Physicochemical properties including swelling, viscoelasticity, and encapsulation efficiency were assessed.
  • Mesenchymal stem cell (MSC) viability and morphology within microcapsules were evaluated over 14 days.
  • Cell release kinetics from microcapsules were monitored over time.

Main Results:

  • Optimized electrospray parameters yielded uniform spherical microcapsules (175.4 ± 21.1 μm) with a microporous architecture.
  • RGD peptide incorporation did not significantly alter alginate swelling, viscoelasticity, or encapsulation efficiency.
  • RAlg microcapsules maintained significantly higher MSC viability (91.3%) compared to Alg alone (84.9%) after 14 days.
  • A time-dependent release profile was observed, with extensive cell release by day 14 (78-81%).

Conclusions:

  • Electrosprayed RAlg microcapsules represent a biocompatible and injectable platform for delivering viable MSCs.
  • This technology enhances MSC viability and provides sustained release, addressing key limitations in cell therapy.
  • The developed microcapsules hold significant potential for applications in regenerative medicine.