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Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Related Experiment Video

Updated: May 9, 2026

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

Integrated bulk and single-cell transcriptomic analyses identify transcriptome-defined groups and EGFR-associated

Yuanhan Wang1, Long Li2, Xing Hu1

  • 1Department of Thoracic Surgery, The Affiliated Cancer Hospital of Guizhou Medical University, No.1 Beijing West Road, Yunyan District, Guiyang City, 550004, Guizhou Province, China.

Discover Oncology
|May 8, 2026
PubMed
Summary

This study reveals two glioma subtypes based on gene expression. The EGFR-high subtype shows proliferative and angiogenic programs, while the EGFR-low subtype exhibits enhanced immune infiltration, guiding precision treatment strategies.

Keywords:
EGFRGliomaMolecular subtypesSingle-cell RNA sequencingTumor microenvironment

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Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

Related Experiment Videos

Last Updated: May 9, 2026

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

Area of Science:

  • Oncology
  • Genomics
  • Molecular Biology

Background:

  • Glioma exhibits significant molecular heterogeneity.
  • Understanding molecular subtypes is crucial for personalized treatment strategies.

Purpose of the Study:

  • To identify transcriptome-defined glioma groups.
  • To characterize cellular heterogeneity and EGFR-associated malignant cell programs using single-cell RNA sequencing.
  • To provide molecular evidence for precision classification and subtype-specific therapeutic strategies.

Main Methods:

  • Integrated bulk and single-cell RNA sequencing (RNA-seq) data (GSE35169, GSE131928).
  • Performed differential gene expression analysis to identify subtype-specific biomarkers.
  • Conducted cell-type annotation, pseudotime trajectory, and cell-cell communication inference.
  • Validated key findings using quantitative real-time PCR (qRT-PCR) in glioma cell lines and normal astrocytes.

Main Results:

  • Identified two transcriptome-defined groups from bulk RNA-seq.
  • Discovered distinct EGFR-associated malignant cell programs in scRNA-seq: EGFR-high cells showed enrichment of extracellular matrix (ECM)-related genes and enhanced proliferative/angiogenic signaling, while EGFR-low cells exhibited higher expression of immune-related genes.
  • Confirmed elevated expression of specific genes (e.g., EGFR, IGFBP2, COL1A1, CXCL10, IL6, STAT1) in corresponding cell lines via qRT-PCR.

Conclusions:

  • Identified bulk transcriptome-defined glioma groups and EGFR-associated malignant cell programs.
  • Linked EGFR-high malignant cells to proliferative and angiogenic programs with specific microenvironmental interactions.
  • Linked EGFR-low malignant cells to enhanced immune infiltration, supporting precision classification and subtype-specific therapeutic strategies.