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Updated: May 10, 2026

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
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Ultrasound-Assisted DES Extraction of Black Rice Anthocyanins: Optimization, Characterization, and Bioactivity.

Cuiqin Li1, Yabin Shi1, Ziming Cao1

  • 1College of Chemistry and Pharmaceutical Engineering, Cangzhou Jiaotong University, Cangzhou, China.

Journal of Food Science
|May 9, 2026
PubMed
Summary

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A novel green extraction method using ultrasound and deep eutectic solvents (UDE) significantly boosts anthocyanin yield from black rice. This efficient UDE process enhances antioxidant and hypoglycemic properties, making black rice a valuable health product.

Area of Science:

  • Food Science and Technology
  • Natural Product Chemistry
  • Biotechnology

Background:

  • Black rice anthocyanins possess significant antioxidant and potential hypoglycemic properties.
  • Low extraction yields currently limit the application of black rice anthocyanins in food industries.
  • Development of efficient and green extraction methods is crucial for value-added applications.

Purpose of the Study:

  • To establish and optimize an ultrasound-assisted deep eutectic solvent extraction (UDE) method for black rice anthocyanins.
  • To characterize the extracted anthocyanins and evaluate their bioactivities.
  • To promote the application of black rice as a value-added health product.

Main Methods:

  • Development of a green ultrasound-assisted deep eutectic solvent extraction (UDE) method.
Keywords:
anthocyaninsantioxidant activityblack ricedeep eutectic solventshypoglycemic activityresponse surface methodologyultrasound‐assisted extraction

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  • Optimization of extraction parameters including solvent composition, solid-to-liquid ratio, water content, and temperature.
  • Analysis of anthocyanin yield, composition (LC-MS), antioxidant activity, and enzyme inhibitory effects (α-glucosidase, α-amylase).

    • Main Results:

    • The optimized UDE method achieved a maximum anthocyanin yield of 37.44 mg/g, significantly higher than ultrasound-assisted ethanol extraction (UEE).
    • Cyanidin-3-glucoside was identified as the predominant anthocyanin component.
    • UDE extracts demonstrated superior antioxidant and inhibitory effects against α-glucosidase and α-amylase compared to UEE extracts.

    Conclusions:

    • The UDE method is an efficient and green technique for extracting high-yield, bioactive anthocyanins from black rice.
    • This optimized protocol addresses the low yield bottleneck, offering a safer alternative to conventional organic solvents.
    • Black rice anthocyanin extracts are suitable for functional foods, dietary supplements for blood glucose management, and natural colorants.