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Updated: May 11, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
Published on: April 29, 2016
A single-cell solution to a transplant mystery
Jana Ihlow1, Samantha D Praktiknjo2
1Institute of Pathology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany; Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Berlin, Germany.
Abstract:
In this Backstory, the authors recount how tailored single-cell sequencing solved a diagnostic puzzle in a young woman who developed myelodysplastic neoplasm after allogeneic hematopoietic stem cell transplantation. Conventional diagnostics could not determine whether the mutant clone was donor- or recipient-derived. Combining transcriptome profiling with targeted U2AF1 enrichment pinpointed the clone's origin and guided a second transplant, leading to remission.
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