A Rapid and Sensitive HPLC-MS/MS Method for Quantification of Propranolol in Rat Plasma and the Application to
Li Bei1, Huang Haijin2, Wan Lili3
1Division of Pharmaceutics, National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
Abstract:
A novel HPLC-MS/MS method has been developed for the determination of propranolol in rat plasma in the present study. The analytes of interest were retained and separated on a C18 column, and a triple-quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source was applied for quantification. A simple protein precipitation method was used to pretreat the plasma; the extraction recovery (ER) was about 100%, and the matrix effect (ME) could be negligible. The method was linear over the concentration ranges of 0.625-2500.00 ng/mL for propranolol. The precision and accuracy were within acceptable limits. The lowest limit of quantification (LLOQ) was 0.625 ng/mL. This simple method was validated and successfully applied to support the pharmacokinetic study after administration of the propranolol transdermal drug delivery system to Sprague-Dawley rats.
