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A Simple and Rapid HPLC-MS/MS Method for Quantification of 15,16-Dihydrotanshinone I in Plasma and the Application to
Lv Dan1, Huang Haijin2, Liu Qian3
1Division of Pharmaceutics, National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
Abstract:
In this study, a rapid and simple HPLC-MS/MS method was developed for the quantification of 15,16-dihydrotanshinone I (DHTS) in rat plasma. DHTS was well retained and separated from the polar endogenous components on a C18 column, and a triple-quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source was applied for quantification. A simple protein precipitation pretreatment method could provide high extraction recovery (from 93.6% to 98.4%) and negligible matrix. The method demonstrated excellent linearity across the concentration range of 0.1~1000.0 ng/mL for DHTS. The precision and accuracy were within acceptable limits. The lowest limit of quantification (LLOQ) was determined to be 0.1 ng/mL. This simple method was validated and successfully applied to support the pharmacokinetics study after DHTS was administered to the Sprague-Dawley rats.
