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Hemoglobin fraction quantitation with Capillarys 2 FP HbA1c mode in patients with known sickle cell disease
Olav Klingenberg1,2, Bente Fjeld1,2, Ingunn B O Lundbye1
1Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
Objective:
To evaluate the feasibility of using Sebia Capillarys HbA1c mode for quantifying hemoglobin fractions in patients with known sickle cell disease to reduce turnaround time.
Methods:
Hemoglobin fractions were analyzed in 58 samples from patients with previously diagnosed sickle cell disease using the Sebia Capillarys 2 FP HbA1c mode (A1c mode) and Sebia Capillarys 2 FP, HEMOGLOBIN(E) mode (Hb mode). Method comparison was performed using Bland-Altman and linear regression analyses. Precision was determined by repeated analysis of internal control material and patient samples, while linearity was studied by serial dilution of a sample containing HbS with a sample without HbS.
Results:
The A1c mode showed good agreement with the Hb mode for quantification of HbA, HbS, and HbF fractions, with small biases of 1.44%, -1.66%, and 0.-02% respectively. Good linearity was demonstrated for HbS and HbA quantification (R2=0.9995).
Conclusion:
The A1c mode is appropriate for quick measurement of hemoglobin fractions in patients with known sickle cell disease, allowing for improved turnaround times in clinical settings. Due to occasional peak shifts and peak mislabeling by the software, a flow chart is included to guide proper A1c mode electropherogram interpretation.
