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Updated: May 15, 2026
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Modeling Ligands into Maps Derived from Electron Cryomicroscopy
Published on: July 19, 2024
Structural Determination and Ligand Interaction of Macrophage Inhibitory Factor: A Standardized X-Ray Crystallography
Emmanuel K Yeboah1, Alessandro T Caputo2
1Immunity and Immune Evasion Laboratory, School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, 3083, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2026
Abstract:
The determination of experimental X-ray crystal structures of macrophage migration inhibitory factor (MIF) with bound ligands is a crucial step in understanding their binding modes and steering medicinal chemistry efforts. Here, a generic method for the crystallization of MIF and subsequent structural analysis with ligands is described.
