Nanozyme-Enhanced Paper-Based Bipolar Electrode Biosensor for Dual-Mode Detection for M918T in Serum with Single-Base...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Molecular Diagnostics

Background:

• The RET M918T mutation is a crucial biomarker for early medullary thyroid carcinoma (MTC) screening.
• Ultralow abundance of M918T mutations poses detection challenges for conventional methods, limiting point-of-care testing (POCT).

Purpose of the Study:

• To develop a sensitive, single-base resolution detection method for the RET M918T mutation.
• To create a dual-modal biosensor for reliable MTC screening and POCT.

Main Methods:

• Designed a paper-based bipolar electrode-electrochemiluminescence (BPE-ECL) and colorimetry dual-modal biosensor.
• Integrated 2'-fluorinated nucleotide (2'-FN) Y-shaped junctions for enhanced mismatch discrimination.
• Utilized g-C3N4@FeMOFs nanozyme for dual amplification of ECL and colorimetric signals.

Main Results:

• Achieved limits of detection of 0.76 pM (ECL) and 1.4 pM (colorimetry) for M918T.
• Demonstrated successful application in human serum samples.
• Validated results through mutual self-validation of dual signals, avoiding false positives.

Conclusions:

• The developed dual-mode biosensor provides a rapid, low-cost, and portable platform for MTC biomarker detection.
• This strategy shows significant potential for early diagnosis and on-site screening of MTC.
• The biosensor enables sensitive and reliable detection of ultralow abundance mutations.