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Updated: May 17, 2026
11:26
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Identification of the Novel HLA-DQB1*03:03:38 Allele Using PolyseqOne Nanopore Sequencing Technology
Weiyuan Chen1,2, Yuqiu Li1, Pingping Zhang1
1Department of Blood Transfusion, Zhongshan Hospital (Xiamen), Fudan University, Xiamen, China.
HLA
|May 15, 2026
Summary
The HLA-DQB1*03:03:38 allele differs from HLA-DQB1*03:03:02:01 by a single nucleotide change. This synonymous substitution occurred in codon 258 of exon 5.
Area of Science:
- * Human Leukocyte Antigen (HLA) genetics
- * Molecular biology
- * Immunology
Background:
- * The HLA system plays a crucial role in immune responses.
- * Specific HLA alleles are associated with various diseases and transplantation outcomes.
- * Detailed characterization of HLA alleles is essential for understanding immune function.
Purpose of the Study:
- * To precisely define the genetic difference between two closely related HLA-DQB1 alleles.
- * To identify the specific nucleotide variation distinguishing HLA-DQB1*03:03:38 from HLA-DQB1*03:03:02:01.
Main Methods:
- * Nucleotide sequencing of relevant HLA-DQB1 gene regions.
- * Comparative sequence analysis to identify variations.
Main Results:
- * HLA-DQB1*03:03:38 and HLA-DQB1*03:03:02:01 differ by a single nucleotide substitution.
- * This variation is a synonymous substitution located in codon 258 of exon 5.
Conclusions:
- * A specific synonymous nucleotide substitution defines the HLA-DQB1*03:03:38 allele.
- * This precise molecular definition aids in HLA typing and immunological studies.
