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Related Experiment Video

Updated: May 20, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

Optimized NMR-Based Metabolomic Protocol for Salivary Analysis in Clinical Practice.

Manon Campas1,2, Constance Germay1,2, Arianna Cirillo1,2

  • 1Clinical Metabolomics Group (CliMe), Centre for Interdisciplinary Research on Medicines (CIRM), University of Liege, Liege 4000, Belgium.

Journal of Proteome Research
|May 18, 2026
PubMed
Summary

This study optimized saliva sample preparation for nuclear magnetic resonance (NMR) metabolomics. An improved method enhances metabolite detection and ensures reliable, quantitative analysis for personalized medicine applications.

Keywords:
clinical metabolomicsintra- and interday precisionnuclear magnetic resonance (NMR)salivasample preparation

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Personalized Medicine

Background:

  • Saliva is an underutilized biofluid in metabolomics compared to blood and urine.
  • Its noninvasive collection and self-sampling suitability make it ideal for clinical applications.
  • Saliva offers complementary metabolic data to other biofluids.

Purpose of the Study:

  • To identify an optimal protocol for saliva sample collection and preparation.
  • To maximize informative metabolomic data from salivary nuclear magnetic resonance (NMR) profiles.
  • To establish a reliable method for quantitative salivary metabolomics.

Main Methods:

  • Systematic comparison of four adapted preparation protocols.
  • Evaluation criteria included metabolite identification, concentration, and intraday repeatability.
  • An in-house method combining centrifugation, freeze-drying, and ultrafiltration was optimized.

Main Results:

  • The optimized protocol quantified 42 metabolites, providing broad metabolome coverage.
  • Analysis of variance confirmed excellent intra- and interday precision (CV < 10%).
  • High-quality spectra and strong analytical precision were achieved.

Conclusions:

  • The optimized protocol supports reproducible salivary NMR metabolomics.
  • Standardized saliva collection and preparation are crucial for its use in diagnostics and disease monitoring.
  • Saliva is a promising biofluid for personalized medicine.