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Updated: May 20, 2026
Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization
Published on: January 17, 2020
Protocol for insulin dosing during expansion and maturation of human iPSC-derived cardiomyocytes
Devin Verbueken1, Qianliang Yuan2, Vincent A J Warnaar3
1Amsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands; Department of Cardiology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands.
Abstract:
Techniques for differentiating human induced pluripotent stem cells (hiPSCs) into cardiomyocytes (hiPSC-CMs) can yield up to 98% purity, yet control over hiPSC-CM fate is lacking. Here, we present a protocol for insulin dosing during the expansion and maturation of hiPSC-derived cardiomyocytes. We describe steps for differentiation, purification, replating, cryopreservation, and thawing. We then detail a procedure for expansion using insulin and Wnt/β-catenin signaling, followed by steps to initiate maturation by either removing insulin completely or adding a low dose of insulin. For complete details on the use and execution of this protocol, please refer to Yuan et al.1.
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