Protocol for establishing an infrared spectral reference library for canine urolith analysis

Canine urolithiasis is a common, recurrent urinary disorder in dogs, posing significant clinical and economic burdens. Breed predispositions suggest genetic and metabolic influences. Accurate determination of urolith composition is essential for effective treatment and prevention. Here, we present a protocol to generate an infrared spectral library of key mineral components for canine urolith analysis. We describe steps for mineral selection, preparation of reference spectra, and library construction. We then detail a workflow for reliable urolith analysis.