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Artificial Humic Acid Derived from Microorganisms Promotes Root Growth of Rice Seedlings by Mediating Microbial
Fuhui Yu1, Bowen Fan2, Hongyu Li1
1College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Abstract:
While artificial humic acid (AHA) benefits soil and crops, the effects of novel microbial-derived AHA on plant root growth remain unclear. This indoor study examined plant-derived, pure microbe-derived, and straw-fed microbial AHA (LPRS-AHA) on rice seedling roots and soil microbial communities. LPRS-AHA showed the strongest effect. At 28 days, compared to CK, LPRS-AHA significantly increased root length (35.6%), surface area (56.2%), volume (112.6%), tip number (37.1%), branches (46.9%), and both root (12.3%) and shoot (14.8%) dry biomass. Possible mechanisms include that LPRS-AHA's nutrient and small-molecule compounds increased the activities of soil N-acetyl-β-d-glucosaminidase, alkaline phosphatase, and l-leucine aminopeptidase, and significantly reshaped the microbial community. Specifically, LPRS-AHA enriched beneficial taxa (e.g., Pseudomonadota phylum, Pseudomonas genus), improved bacterial adaptability and fungal stability, and intensified plant-microbe interactions. This study provides foundational evidence for the development of microbial-derived AHA in rice seedling cultivation.
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