Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 23, 2026

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
08:31

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

A deterministic method for quantifying spindle-shaped cells in noisy bright-field microscopy.

Martin Radvanský1, Markéta Vašinková2, Miloš Kudělka1

  • 1Department of Computer Science, FEECS, VSB - Technical University of Ostrava, 17. listopadu 2172/15, 70800, Ostrava, Czech Republic.

Scientific Reports
|May 21, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulatory function of ten‑eleven translocation‑2 in transcriptional mechanisms of demethylated myeloma cells.

Molecular medicine reports·2026
Same author

Contribution of host-related immune factors to frequency and severity of infections in lung transplant recipients.

Respiratory research·2026
Same author

Severe Hypertension and Hematologic Abnormalities in Pseudoxanthoma Elasticum: A Pediatric Case Report.

Journal of clinical hypertension (Greenwich, Conn.)·2026
Same author

GAN-based bone suppression using a combined loss function.

Frontiers in artificial intelligence·2026
Same author

[COVID-19 pandemic in a cohort of lung transplant recipients: Insights into immune system status].

Klinicka mikrobiologie a infekcni lekarstvi·2026
Same author

A novel host-based strategy for detecting low-grade prosthetic joint infection using immune cell pattern in synovial fluid.

Molecular medicine (Cambridge, Mass.)·2026

A new image analysis method accurately quantifies spindle-shaped cells in bright-field microscopy without fluorescent stains or deep learning. This deterministic approach offers a practical solution for analyzing cell populations in challenging imaging conditions.

Area of Science:

  • Cell Biology
  • Biomedical Imaging
  • Computational Biology

Background:

  • Accurate quantification of spindle-shaped cells in bright-field microscopy is difficult due to low contrast, noise, and variable morphology.
  • Conventional methods like fluorescent staining or deep learning have limitations, including phototoxicity, extensive data requirements, and lack of interpretability.

Purpose of the Study:

  • To develop a deterministic image analysis method for accurate spindle-shaped cell quantification directly from bright-field microscopy images.
  • To provide a practical alternative to fluorescent labeling and deep learning for analyzing cell populations.

Main Methods:

  • The workflow integrates contrast enhancement, adaptive thresholding, contour filtering, and shape-guided refinement.
  • The method is designed to be robust against irregular cell shapes and heterogeneous backgrounds in time-lapse microscopy.
Keywords:
Bright-field microscopyCell countingContour detectionFibroblast-like cellsGaussian-based modelSpindle-shaped cells

More Related Videos

Automated Quantification of Synaptic Fluorescence in C. elegans
12:22

Automated Quantification of Synaptic Fluorescence in C. elegans

Published on: August 10, 2012

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins
11:01

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Published on: November 17, 2016

Related Experiment Videos

Last Updated: May 23, 2026

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
08:31

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

Automated Quantification of Synaptic Fluorescence in C. elegans
12:22

Automated Quantification of Synaptic Fluorescence in C. elegans

Published on: August 10, 2012

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins
11:01

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Published on: November 17, 2016

  • No fluorescent labeling or supervised training is required.

    • Main Results:

    • The method reliably quantifies spindle-shaped cells under challenging imaging conditions, including noise and low contrast.
    • Consistent performance was observed across varying noise levels and imaging parameters.
    • The approach achieved competitive accuracy with high interpretability and low computational cost.

    Conclusions:

    • This deterministic image analysis method offers a practical and interpretable solution for large-scale spindle-shaped cell quantification in bright-field microscopy.
    • It avoids the drawbacks of fluorescent staining and complex deep learning models.
    • Enables straightforward integration into existing biomedical imaging workflows.