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Updated: May 23, 2026
Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.
Published on: March 25, 2020
Effect of Acellular Adipose Matrix on Wound Regeneration in a Murine Model
Jaewoo Kim1,2, Vinh Vuong The Tran1,3, Dongjian Li1
1Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea.
Background:
Chronic and acute wounds pose major challenges in clinical practice. Current treatments offer therapeutic benefits but are limited by high cost, technical complexity, and limited availability. The acellular adipose matrix (AAM), derived from decellularized human adipose tissue, contains extracellular matrix components and bioactive molecules that may promote tissue repair and angiogenesis. In this study, we evaluated the therapeutic potential of AAM in promoting wound healing in a murine model.
Methods:
Human abdominal adipose tissue was processed into AAM through mechanical, chemical, and enzymatic decellularization. Bilateral full-thickness dorsal wounds (1 cm in diameter) were created in nude mice. The control group received an antibiotic ointment, while the experimental group received AAM. Wound closure was monitored for 16 days. Histological and immunofluorescence analyses were performed to assess epithelialization, collagen remodeling, and angiogenesis. Statistical analysis was performed using the Mann-Whitney U test, with p < 0.05 considered significant.
Results:
The AAM-treated group exhibited faster epithelial coverage and granulation tissue formation than the control group. The mean healing time was significantly shorter in the AAM group (9.0 ± 1.67 days) compared with controls (12.0 ± 1.79 days). CD31 staining confirmed the presence of dense microvascular networks and significantly enhanced angiogenesis. Collagen fibers were evenly organized without abnormal fibrosis in both groups.
Conclusions:
AAM effectively enhanced wound regeneration and angiogenesis in vivo. Its biological activity, accessibility, and potential for cost-effective production suggest that AAM may serve as a clinically applicable "off-the-shelf" biomaterial for wound management in both reconstructive and aesthetic surgeries.
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