Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 26, 2026

Automated High-throughput Behavioral Analyses in Zebrafish Larvae

Automated High-throughput Behavioral Analyses in Zebrafish Larvae

Published on: July 4, 2013

AquaMaze: A Computer Vision-Based Framework for Automated Behavioral Analysis of Zebrafish in Controlled

Ali Samet Ayık¹, Ceren Aydoğan^2,3, Bora Deniz Yılmaz^2,3

¹Ostim Technical University, Ankara, Türkiye.

|May 24, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The efficacy of modified sternocleidomastoid muscle flap in preventing sialocele and salivary fistula following partial superficial parotidectomy.

Acta oto-laryngologica·2026

Same author

The effect of tumor budding index on clinical parameters in early-stage oral cavity cancers.

Acta oto-laryngologica·2026

Same author

Relationship Between the Lactate-to-Albumin Ratio and Acute Kidney Injury in Patients with Pulmonary Embolism: A Retrospective Cohort Study.

Medicina (Kaunas, Lithuania)·2026

Same author

Exploratory analysis of stromal and peritumoural tumour-infiltrating lymphocytes (tils) in malignant parotid gland tumours: associations with clinicopathological factors and survival.

Acta oto-laryngologica·2026

Same author

The relationship between P-wave peak time and albuminuria in hypertensive patients.

Postgraduate medicine·2026

Same author

The role of nitric oxide in hypertensive target organ damage in patients without renal impairment: insights from left ventricular global longitudinal strain and albuminuria.

BMC cardiovascular disorders·2026

AquaMaze is a new, free software for tracking zebrafish behavior in neuroscience research. This open-source tool provides reliable locomotion metrics for high-throughput behavioral assays.

Area of Science:

Behavioral neuroscience
Pharmacological studies
Zebrafish model organism research

Background:

Zebrafish (Danio rerio) are valuable model organisms in neuroscience and pharmacology.
Current manual or semi-automated tracking systems are often expensive and labor-intensive.
Limited budgets hinder access to advanced behavioral analysis tools for many researchers.

Purpose of the Study:

Introduce AquaMaze, an open-source, desktop application for zebrafish behavioral tracking.
Provide a cost-effective and scalable solution for real-time and post-hoc behavioral analysis.
Facilitate high-throughput behavioral assays in research settings.

Main Methods:

AquaMaze is a standalone application developed using PyQt5 and OpenCV.

Keywords:

behavior novel object recognition novel tank test swim behavior test y-maze zebrafish

More Related Videos

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Published on: December 5, 2013

Development of automated imaging and analysis for zebrafish chemical screens.

Development of automated imaging and analysis for zebrafish chemical screens.

Published on: June 24, 2010

Related Experiment Videos

Last Updated: May 26, 2026

Automated High-throughput Behavioral Analyses in Zebrafish Larvae

Automated High-throughput Behavioral Analyses in Zebrafish Larvae

Published on: July 4, 2013

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Published on: December 5, 2013

Development of automated imaging and analysis for zebrafish chemical screens.

Development of automated imaging and analysis for zebrafish chemical screens.

Published on: June 24, 2010

The software analyzes user-defined video inputs to compute locomotion metrics.

Key metrics include swim distance, speed, quadrant occupancy, and rest/activity patterns.

Main Results:

AquaMaze provides intuitive visualizations like heatmaps.
Detailed CSV reports are generated for comprehensive data analysis.
The software demonstrated reliable and consistent results across various behavioral paradigms.

Conclusions:

AquaMaze offers a fast, cheap, and reliable tool for zebrafish behavioral experiments.
The open-source nature promotes accessibility for researchers with limited budgets.
This application supports scalable, high-throughput behavioral assays in neuroscience research.