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Updated: May 28, 2026

A Practical Guide to Phage- and Robotics-Assisted Near-Continuous Evolution
Published on: January 12, 2024
Advances in protein engineering
Huong Lan Vuong1, Hien Thi Thu Le2
1National Hospital for Tropical Disease, Pharmacy Department, Hanoi, Vietnam.
Abstract:
Protein engineering (PE) has been applied to various medicines, food, and environments. Contributions of proteins have been reported with remarkable results in protein therapeutics, antibody engineering, enzyme synthesis, and more specific functions in industrial processes. Therefore, this chapter highlights the most recent PE advances in a battle against mainly human diseases and biomedical sciences. The application of PE will be reviewed, focusing on developing innovative techniques, including evolution, rational design, semi-rational design, and hybrid approaches to protein design in applications. In addition, we provide key achievements of PE in CRISPR/Cas systems, high-through data, and synthetic biology with updated results. Current challenges of using PE, ethical considerations, and various approaches for protein therapeutics are also discussed. In this chapter, the updated findings provide a comprehensive overview of the transformative potential of PE for researchers in the application areas of human disease, especially in cancer therapeutics.
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