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A Novel Hydrophilic Colloidal Polysaccharide from Rosa roxburghii Tratt: Structural Characterization, Rheological
Chenxi Cui1,2,3, Miao Wang1,2,3, Qiuli Zhang1,2,3
1State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Foods (Basel, Switzerland)
|May 27, 2026
Summary
A novel polysaccharide, RTW-1, from Rosa roxburghii fruit demonstrates significant immunostimulatory effects. This functional food ingredient shows potential for enhancing intestinal immunity and promoting macrophage activity.
Area of Science:
- Food Science
- Biochemistry
- Immunology
Background:
- Rosa roxburghii Tratt is recognized for its nutritional and medicinal value.
- Polysaccharides are key bioactive compounds in Rosa roxburghii.
Purpose of the Study:
- To extract and characterize a homogeneous polysaccharide (RTW-1) from Rosa roxburghii fruit.
- To evaluate the biological activities, including immunostimulatory properties, of RTW-1.
Main Methods:
- High-performance liquid chromatography (HPLC) for molecular mass determination.
- Monosaccharide composition, methylation analysis, and uronic acid content measurement.
- Rheological analysis, macrophage assays (proliferation, phagocytosis, NO production), and qRT-PCR for cytokine mRNA expression.
Main Results:
- RTW-1 has a molecular mass of 2.16 × 10^3 kDa and is primarily composed of glucose, arabinose, and galacturonic acid.
- RTW-1 exhibits shear-thinning behavior and favorable gel-forming properties.
- RTW-1 significantly promotes RAW264.7 macrophage proliferation, phagocytic activity, and nitric oxide production, and upregulates key inflammatory cytokine mRNA expression in a dose-dependent manner.
Conclusions:
- RTW-1 possesses significant immunostimulatory properties.
- RTW-1 demonstrates potential as a functional food ingredient for promoting intestinal immune activity.
