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Related Experiment Video

Updated: May 28, 2026

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
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Published on: November 3, 2023

Label-Free Optical Sensor for Real-Time Monitoring of Insulin Secretion from Single Human Pancreatic Islets.

Mark F Coughlan1, Lei Zhang1, Umar Khan1

  • 1Center for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02115, USA.

Sensors (Basel, Switzerland)
|May 27, 2026
PubMed
Summary

This study introduces an islet-on-a-chip sensor using advanced microscopy to monitor insulin secretion from single human islets in real-time. This label-free method enables continuous, non-destructive assessment of islet function, crucial for diabetes research.

Keywords:
insulinislet-on-a-chiplabel-freemicroscopy

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Last Updated: May 28, 2026

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Area of Science:

  • Biomedical Engineering
  • Endocrinology
  • Cell Biology

Background:

  • Pancreatic islets are vital for glucose homeostasis, with insulin secretion being a key function.
  • Current methods for assessing insulin secretion often lack real-time, single-islet resolution.
  • Limitations include offline processing and the need to pool multiple islets, hindering dynamic analysis.

Purpose of the Study:

  • To develop a novel, label-free biosensor for continuous, real-time monitoring of insulin secretion dynamics in individual human islets.
  • To enable high-resolution optical interrogation of single islets without destructive procedures.
  • To support longitudinal studies of islet function and dysfunction.

Main Methods:

  • Fabrication of an islet-on-a-chip (IOC) device using two-photon polymerization.
  • Integration of broadband backscattering confocal microscopy (BBCM) for label-free optical readout.
  • Stabilization of single human islets under continuous perfusion for real-time monitoring.

Main Results:

  • The IOC-BBCM sensor successfully identified insulin-rich beta cells label-free.
  • It monitored granule depletion and redistribution during glucose and potassium chloride stimulation.
  • Results correlated with ELISA-quantified insulin secretion from the same islets.

Conclusions:

  • The developed modular sensor offers a non-destructive, label-free approach for monitoring stimulus-linked secretion dynamics from individual human islets.
  • This technology facilitates real-time assessment of single-islet function, overcoming limitations of existing assays.
  • The sensor holds promise for advancing research in diabetes and islet biology.