Oleyl alcohol-enhanced embedding resin for histological analysis of non-decalcified hard tissue

Several acrylate-based materials are commercially available as embedding resins for the histological analysis of non-decalcified hard tissues, including implants. However, commercial embedding resins are imported, expensive, and take a long time to buy. We conducted several tests to apply a mixture of methyl methacrylate and oleyl alcohol (oleyl Alc) as an embedding resin on a non-decalcified bone specimen. Further, we observed differences in the compressive strength of the block resin and the fused bone sample, changes in crystallinity, and a hydrophilic change in the block. Hematoxylin and eosin (H&E) and Masson's Trichrome (MT) staining were performed for the histological analysis of dog bones to compare staining characteristics. The block to which the resin with the optimal composition ratio was applied for embedding was confirmed experimentally to achieve optimal performance.