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Updated: May 31, 2026

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Monitoring Cell Membrane Hydration Using a Fluorescent Probe Sensitive to Trace Water.

Zixu He1,2, Diankai Liu1, He Li1

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.

Advanced Materials (Deerfield Beach, Fla.)
|May 29, 2026
PubMed
Summary

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This summary is machine-generated.

Researchers developed a new fluorescent probe, RMDR (rhodamine-derivatized membrane deep red), to visualize cell membrane hydration. This probe tracks water content changes, aiding studies of cell membrane dynamics in various physiological and stress conditions.

Area of Science:

  • Cell biology
  • Biophysics
  • Chemical biology

Background:

  • Cell membrane hydration is crucial for membrane structure and function.
  • Directly observing cell membrane hydration is difficult due to a lack of specific probes.

Purpose of the Study:

  • To develop a novel fluorescent probe for visualizing and quantifying cell membrane hydration.
  • To investigate dynamic changes in cell membrane hydration in response to stimuli.

Main Methods:

  • Development of RMDR (rhodamine-derivatized membrane deep red), a membrane-localized fluorescent probe.
  • Utilizing fluorescence lifetime imaging microscopy (FLIM) to detect polarity-dependent changes.
  • Monitoring membrane hydration in living cells under hypotonic stimulation, cuproptosis, and lipid peroxidation.
Keywords:
cell membranecuproptosisfluorescence lifetime microscopyfluorescent probemembrane hydration

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Main Results:

  • RMDR shows decreased fluorescence lifetime with increased local water content (0-1%).
  • Visualized increased cell membrane hydration upon hypotonic stimulation.
  • Identified spatial-temporal hydration gradients in cell clusters.
  • Observed increased membrane hydration during cuproptosis and lipid peroxidation.

Conclusions:

  • RMDR is an effective tool for examining membrane hydration dynamics in living cells.
  • This probe facilitates research into water-associated cellular processes in physiological and stress-related contexts.