Related Experiment Video
Updated: Jun 3, 2026
09:50
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Light-Dependent Regulation of Flavonol Biosynthesis through the HY5-MYB-FLS Module in Walnut
Na Wang1,2, Yi Jiang1,2, Erjuan Chen1,2
1College of Agriculture, Guizhou Engineering Research Center for Fruit Crops, Guizhou University, Guiyang 550025, China.
Journal of Agricultural and Food Chemistry
|June 2, 2026
Summary
High light activates flavonol biosynthesis in Juglans sigillata via the JsHY5-JsMYB141-JsFLS4 pathway. This transcription module links light signals to secondary metabolism in woody plants.
Area of Science:
- Plant molecular biology
- Plant biochemistry
- Environmental regulation of plant metabolism
Background:
- Light is a critical environmental factor influencing plant secondary metabolism.
- Flavonol biosynthesis is a key pathway regulated by light.
- The R2R3-MYB transcription factor JsMYB141 is implicated in Juglans sigillata flavonol production.
Purpose of the Study:
- To elucidate the transcriptional mechanism by which light signals modulate flavonol biosynthesis in Juglans sigillata.
- To investigate the roles of JsMYB141 and its potential regulators in response to light.
- To understand the JsHY5-JsMYB141-JsFLS4 regulatory module.
Main Methods:
- Gene expression analysis under high light conditions.
- Homologous and heterologous functional validation of JsMYB141 and JsFLS4.
- Overexpression studies of JsHY5.
- Molecular interaction assays to assess transcriptional activation.
Main Results:
- High light induces the expression of JsMYB141 and its target gene JsFLS4.
- JsMYB141 and JsFLS4 significantly enhance flavonol biosynthesis under high light.
- Overexpression of JsHY5 upregulates JsMYB141 and boosts flavonol production.
- JsHY5 and JsMYB141 cooperatively activate JsFLS4 transcription.
Conclusions:
- The JsHY5-JsMYB141-JsFLS4 module is crucial for connecting light signaling to flavonol biosynthesis in Juglans sigillata.
- This study establishes a foundational framework for understanding light-mediated secondary metabolism regulation in woody plants.
Related Concept Videos
01:25Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
01:17Gene Regulation During Sporulation
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
02:04Regulation of Transpiration by Stomata
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
02:00Photoreceptors and Plant Responses to Light
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
02:45Biological Clocks and Seasonal Responses
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
02:16Light Acquisition
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.