Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Precipitate Formation and Particle Size Control01:16

Precipitate Formation and Particle Size Control

In precipitation gravimetry, the precipitating agent should react specifically or selectively with the analyte. While a specific reagent reacts with the analyte alone, a selective reagent can react with a limited number of chemical species.
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Subjective reasoning for persistent worry about radiation seven years after the Fukushima nuclear disaster: a qualitative analysis.

BMC public health·2026
Same author

Overview of the Japanese mental health services through legislation, policies and recent initiatives: a narrative review.

International review of psychiatry (Abingdon, England)·2025
Same author

Surface-Enhanced Raman Scattering Properties of Densely-Packed Thin-Films Fabricated using Mixed Gold Nanoparticles with Different Diameters.

Chemphyschem : a European journal of chemical physics and physical chemistry·2025
Same author

Mass Spectrometry Imaging of Time-Dependently Photodegraded Light Stabilizers in Polyethylene Films Using Tapping-Mode Scanning Probe Electrospray Ionization.

Mass spectrometry (Tokyo, Japan)·2025
Same author

Mental health and psychiatry in Japan.

BJPsych international·2025
Same author

Social networks and posttraumatic stress symptoms five to ten years after the Fukushima nuclear power plant disaster.

Social science & medicine (1982)·2024
Same journal

Synergistic Visible-Light-Driven CO<sub>2</sub> Reduction and H<sub>2</sub>O Oxidation over Ti<sub>3</sub>C<sub>2</sub> Quantum Dot-Modified Cu/g-C<sub>3</sub>N<sub>4</sub> Photocatalysts.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Spontaneous Phase Separation Enables Rapid, Polymerization-Free Fabrication of Gels.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Lamellar-Confinement-Induced ZIF-67 Nanosheet Mixed Matrix Membranes for Enhanced CH<sub>4</sub>/N<sub>2</sub> Separation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Structure Control of Oblate Nanoparticles Self-Assembled by ABC Cyclic Terpolymers under Soft Confinement.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Tuning Brønsted/Lewis Acid Site Ratios via Ammonia Modulation for Selective Conversion of Glycerol to 1,3-Propanediol or Solketal.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Catalytic and Nitriding Competition of Nitrogen Atom on Graphene and Its Finite Rate Surface Chemistry Model.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

Tangential Flow Ultrafiltration: A &ldquo;Green&rdquo; Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
12:47

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Published on: October 4, 2012

Stepwise Centrifuge Classification Method for Citrate-Reduced Silver Nanoparticles To Decrease Size Variance.

Natsuki Koyama1, Takeo Oku2, Tsuyoshi Akiyama2

  • 1Division of Advanced Engineering Science, Graduate School of Engineering, The University of Shiga Prefecture, 2500, Hassaka, Hikone, Shiga 522-8533, Japan.

Langmuir : the ACS Journal of Surfaces and Colloids
|June 3, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a stepwise centrifugal classification method to control silver nanoparticle (AgNP) size without extra stabilizers. This technique enhances AgNP uniformity for improved surface-enhanced Raman scattering (SERS) applications.

More Related Videos

Preparation of Liquid-exfoliated Transition Metal Dichalcogenide Nanosheets with Controlled Size and Thickness: A State of the Art Protocol
10:41

Preparation of Liquid-exfoliated Transition Metal Dichalcogenide Nanosheets with Controlled Size and Thickness: A State of the Art Protocol

Published on: December 20, 2016

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis
06:24

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

Published on: September 13, 2020

Related Experiment Videos

Last Updated: Jun 4, 2026

Tangential Flow Ultrafiltration: A &ldquo;Green&rdquo; Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
12:47

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Published on: October 4, 2012

Preparation of Liquid-exfoliated Transition Metal Dichalcogenide Nanosheets with Controlled Size and Thickness: A State of the Art Protocol
10:41

Preparation of Liquid-exfoliated Transition Metal Dichalcogenide Nanosheets with Controlled Size and Thickness: A State of the Art Protocol

Published on: December 20, 2016

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis
06:24

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

Published on: September 13, 2020

Area of Science:

  • Materials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Silver nanoparticles (AgNPs) are crucial for surface-enhanced Raman scattering (SERS) applications.
  • Controlling AgNP size and shape during synthesis is difficult without stabilizers.
  • Existing methods often require additional stabilizing agents, limiting applications.

Purpose of the Study:

  • To evaluate a stepwise centrifugal classification method for AgNP size control.
  • To demonstrate the applicability of this method for producing uniform AgNPs.
  • To enable AgNP applications in sensitive detection techniques like SERS.

Main Methods:

  • Mathematical modeling of particle classification.
  • Stepwise centrifugal classification of AgNPs synthesized with trisodium citrate.
  • Varying centrifugation time and speed to achieve separation.
  • Redispersing AgNP precipitate using citrate ions to prevent loss.

Main Results:

  • Successful separation of AgNPs into different size distributions.
  • Achieved improved AgNP uniformity through multi-step centrifugation.
  • Maintained AgNP integrity and prevented loss using citrate ions.
  • Produced AgNPs suitable for Raman scattering measurements without additional stabilizers.

Conclusions:

  • Stepwise centrifugal classification is an effective method for controlling AgNP size and uniformity.
  • This stabilizer-free approach enhances AgNP suitability for SERS and other sensitive detection methods.
  • The method offers a pathway to improved spectral resolution in analytical applications.