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Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
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Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

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Progressive embedding technique for large auricular cartilage-containing specimens in Mohs micrographic surgery

Thiago Rubim Bellott1,2, Felipe Bochnia Cerci3,4, Stanislav N Tolkachjov5,6,7,8

  • 1Department of Dermatology, Fluminense Federal University, Niterói, Brazil.

JAAD International
|June 8, 2026
PubMed
Summary

No abstract available in PubMed .

Keywords:
Mohs micrographic surgerydermatologic surgeryfrozen sectionhistologymethodlaboratorypathologyprocessingquality assurancequality controlsingle sectionskin cancer

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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