An ultrasensitive aptamer-based fluorescent biosensor for luteinizing hormone with mutually orthogonal DNAzyme and...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Molecular Diagnostics

Background:

• Sensitive detection of luteinizing hormone (LH) is crucial for disease diagnosis and managing physiological functions.
• Existing methods may lack the required sensitivity or specificity for early disease detection.

Purpose of the Study:

• To develop a novel sensing platform for ultrasensitive luteinizing hormone (LH) detection.
• To harness a synergistic amplification strategy for enhanced analytical performance.

Main Methods:

• Developed a sensing platform utilizing DNAzyme and self-replicating catalytical hairpin assembly (SR-CHA).
• The platform employs an aptamer-based approach for LH recognition.
• Employs a cascade amplification strategy involving DNAzyme and SR-CHA for signal enhancement.

Main Results:

• Achieved an ultrasensitive detection limit for LH as low as 0.00038 mIU/mL in buffer and 0.0006 mIU/mL in real samples.
• Demonstrated a broad dynamic range for LH detection (0.001 to 100 mIU/mL).
• Exhibited excellent selectivity and strong correlation with the ELISA method.

Conclusions:

• The developed platform offers a simple, sensitive, and specific method for LH detection.
• Shows significant potential for constructing various aptamer-based biosensors.
• Provides a promising tool for early disease diagnosis and monitoring physiological functions.