Elucidating the effect of surface disinfection methods on strawberry microbiome composition and volatile organic...

Strawberries are highly perishable, with a shelf life of only a few days at room temperature. It is mostly ascribed to their susceptibility to spoilage, leading to significant postharvest losses. This study employed a unique system to examine the spoilage of strawberries through the combined perspectives of volatilomics and microbiome analysis. Three surface disinfection techniques: UV irradiation, washing with NaClO and ethanol, or with tea waste extract, were compared to untreated samples at temperatures of 4 °C, 10 °C, and 25 °C, respectively. The results indicate that certain volatile organic compounds (VOCs) were affected by the surface disinfection methods and could be correlated with the spoilage severity over time. For each experimental condition, five putative compounds were tentatively identified as key VOCs by statistical analysis, supporting the feasibility of establishing a multidimensional VOC fingerprint matrix for strawberry spoilage monitoring. The fungal microbiota's succession was strongly influenced by temperature, with key spoilage genera like Penicillium, Alternaria, and Cladosporium identified by sequencing, revealing temperature-modulated microbial dynamics. Relationships between identified potential indicators and spoilage microorganisms were elucidated by correlation analysis. The results suggested surface treatments may affect the occurrence of microbial communities, highlighting the complexity of microbial-chemical dynamics during spoilage processes, where VOC profiles appear to function both as metabolic fingerprints and ecological regulators within the spoilage microbiome. These findings provide new insights into the largely unknown metabolic processes involved in strawberry spoilage, offering new opportunities for more effective prevention strategies to reduce spoilage and waste.