Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

ZEB1-AS1 and miR-200b-3p Expression in Non-Muscle-Invasive Bladder Cancer: Pathological Correlates.

Enes Degirmenci¹, Oner Sanli², Murat Kaytaz³

¹Department of Urology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey.

Journal of Clinical Medicine

|June 12, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Can urinary exosomal lncRNAs HOTAIR and MALAT1 predict surgical need and outcomes in unilateral antenatal hydronephrosis?

Pediatric surgery international·2026

Same author

Does WHO/ISUP grade IV histology provide prognostic insight in chromophobe renal cell carcinoma?

International urology and nephrology·2026

Same author

What should the urologist know on the management of kidney cancer in patients with Von Hippel-Lindau syndrome? Recommendations of the European Association of Urology (EAU) Young Academic Urologists (YAU) Renal Cancer Working Group.

World journal of urology·2026

Same author

Association of Ascending Aortic Aneurysm with NOX4 and miRNA 146a.

Genes·2026

Same author

Clinicopathologic features and outcomes of patients with DNAJB9-positive fibrillary glomerulonephritis.

Clinical kidney journal·2026

Same author

Serum human epididymis protein 4, endocan, sialyl-Tn, and neopterin in endometrial cancer: a prospective case-control study.

Revista da Associacao Medica Brasileira (1992)·2026

Same journal

Evidence-Based Clinical Recommendations for the Appropriate Use of Diagnostic Tests in Pediatric Allergology: Focus on Asthma, Rhinoconjunctivitis, and Keratoconjunctivitis Vernal.

Journal of clinical medicine·2026

Same journal

Surgical and Transcatheter Approach of a Failed Mitral Valve Repair: A Comprehensive Review on Selecting the Most Suitable Approach.

Journal of clinical medicine·2026

Same journal

Hybrid Metaheuristic Feature Selection for Breast Cancer Detection in Digital Mammography: A Feasibility Study with Nested Validation, Benchmarking, and External Stress Testing.

Journal of clinical medicine·2026

Same journal

Identity Transformation and the Role of Accountability in Recovery from Problematic Pornography Use: A Phenomenological-Hermeneutical Study.

Journal of clinical medicine·2026

Same journal

Does Early Surgical Treatment in Degenerative Cervical Myelopathy Have a Favorable Clinical Outcome and Impact on Quality of Life?

Journal of clinical medicine·2026

Same journal

Shear Wave Elastography in Musculoskeletal Imaging: A Narrative Review.

Journal of clinical medicine·2026

See all related articles

This summary is machine-generated.

Long non-coding RNA ZEB1-AS1 and microRNA miR-200b-3p showed distinct expression patterns in non-muscle-invasive bladder cancer (NMIBC). These non-coding RNAs are linked to tumor grade, offering insights into epithelial-mesenchymal transition (EMT) pathways.

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are key regulators of epithelial-mesenchymal transition (EMT) and cancer progression.
Understanding the roles of specific non-coding RNAs like ZEB1-AS1 and miR-200b-3p in non-muscle-invasive bladder cancer (NMIBC) is crucial for advancing treatment strategies.

Purpose of the Study:

To investigate the expression profiles of lncRNA ZEB1-AS1 and miR-200b-3p in NMIBC tissues.
To determine the association between the expression levels of ZEB1-AS1 and miR-200b-3p and clinicopathological characteristics of NMIBC.
To evaluate the diagnostic potential of ZEB1-AS1 and miR-200b-3p in NMIBC.

Main Methods:

Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure ZEB1-AS1 and miR-200b-3p expression in 50 NMIBC tissues and adjacent normal bladder tissues.

Keywords:

biomarkers bladder cancer long non-coding RNA microRNA non-muscle-invasive bladder cancer

More Related Videos

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

Related Experiment Videos

Last Updated: Jun 13, 2026

Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

The 2^-ΔΔCT method was employed for relative expression level calculation.

Non-parametric statistical tests and Receiver Operating Characteristic (ROC) curve analysis were utilized to assess associations and diagnostic performance.

Main Results:

Both ZEB1-AS1 and miR-200b-3p exhibited significantly higher expression in NMIBC tissues compared to normal tissues (p=0.018 and p=0.034, respectively).
ZEB1-AS1 expression was notably higher in high-grade tumors (p=0.007), while miR-200b-3p was more prevalent in low-grade tumors (p=0.015).
No significant correlations were found between expression levels and tumor stage, carcinoma in situ, or variant histology. ROC analysis indicated modest diagnostic utility for both markers (AUCs 0.637 and 0.624).

Conclusions:

ZEB1-AS1 and miR-200b-3p display distinct expression patterns correlated with NMIBC tumor grade.
These non-coding RNAs may contribute to the molecular heterogeneity of EMT in NMIBC.
While individual diagnostic performance is limited, further research in larger cohorts is warranted to explore their role in NMIBC pathogenesis and potential as biomarkers.