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Related Experiment Video

Updated: Jun 13, 2026

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

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A genetic tool targeting brain GPR75.

Emma C Wheeler, Runzhou Yang, Stephen M Farmer

    Biorxiv : the Preprint Server for Biology
    |June 12, 2026
    PubMed
    Summary

    G-protein-coupled receptor 75 (GPR75) is key in diet-induced obesity. Researchers developed a new mouse model to map GPR75

    Area of Science:

    • Neuroscience
    • Genetics
    • Metabolism

    Background:

    • G-protein-coupled receptor 75 (GPR75) plays a role in diet-induced obesity (DIO).
    • The precise location of GPR75 within the brain is currently unknown, limiting research into its function in DIO.
    • Understanding GPR75's brain distribution is crucial for developing anti-obesity therapies.

    Purpose of the Study:

    • To generate a novel GPR75-GFP-Ires-Cre knockin mouse model.
    • To investigate the brain-wide expression pattern of GPR75.
    • To establish a tool for studying the neural mechanisms underlying GPR75's role in DIO.

    Main Methods:

    • Generation of a GPR75-GFP-Ires-Cre knockin mouse strain.
    • Confirmation of Cre and GFP colocalization with endogenous GPR75 expression.

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  • Assessment of the GPR75-GFP fusion protein's functionality and DIO susceptibility.
  • Analysis of GPR75 expression throughout the brain using the new mouse model.

    • Main Results:

    • A GPR75-GFP-Ires-Cre knockin mouse model was successfully created.
    • The GPR75-GFP fusion protein is functional and does not alter DIO susceptibility.
    • GPR75 exhibits broad expression across the brain.
    • GPR75 is primarily localized within the cytoplasm of brain neurons.

    Conclusions:

    • The novel GPR75-GFP-Ires-Cre mouse line is a valuable tool for neuroscience research.
    • This model enables detailed investigation of GPR75's neural basis in mediating DIO.
    • The findings provide a foundation for exploring GPR75 as a therapeutic target for obesity.