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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Jun 14, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Ultra-rapid cryo-EM data acquisition method enabled by continuous recording based beam image shift.

Qi Yang1,2, Xiaojun Huang3,4,5, Chunling Wu1

  • 1State Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Nature Communications
|June 12, 2026
PubMed
Summary
This summary is machine-generated.

Continuous Recording Beam-Image Shift (CR-BIS) accelerates cryo-electron microscopy (cryo-EM) data collection by eliminating camera delay. This method enhances efficiency for both single-particle analysis and cryo-electron tomography without hardware changes.

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Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
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Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Related Experiment Videos

Last Updated: Jun 14, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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Published on: March 19, 2021

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Area of Science:

  • Structural Biology
  • Microscopy Techniques

Background:

  • Cryo-electron microscopy (cryo-EM) data acquisition is a bottleneck for high-resolution structure determination.
  • Current methods require significant time for data collection, limiting throughput.

Purpose of the Study:

  • To introduce and validate a novel data acquisition method, Continuous Recording Beam-Image Shift (CR-BIS).
  • To accelerate cryo-EM data collection while maintaining high data quality.

Main Methods:

  • Implemented CR-BIS, a continuous recording strategy during beam-image shift acquisition.
  • Utilized K3 and Falcon 4 direct electron detectors under conventional conditions.
  • Applied CR-BIS to both single-particle analysis and cryo-electron tomography workflows.

Main Results:

  • Achieved acquisition of ~34,000 micrographs per 24h in single-particle analysis mode.
  • Acquired ~1,000 tilt series per 24h in cryo-electron tomography mode.
  • Demonstrated that CR-BIS accelerates data collection without compromising the quality of 3D reconstructions.

Conclusions:

  • CR-BIS significantly enhances the efficiency of cryo-EM data acquisition.
  • The method is broadly applicable, compatible with existing software via scripting, and requires no hardware modifications.
  • CR-BIS facilitates efficient high-resolution cryo-EM studies.