A novel CRISPR/Cas14a-synergized wood-based platform for the ultrasensitive detection of aflatoxin B1
Related Experiment Video
Updated: Jun 17, 2026
Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4
Published on: November 14, 2025
Huixin Chen1, Qing Li1, Xun Mao1
1College of Chemistry and Chemical Engineering, Central South University of Forestry and Technology, Changsha, 410004, P.R. China. xunmao@nwu.edu.cn.
Abstract:
A CRISPR/Cas14a-synergized wood-based platform (CWP) is designed here to detect aflatoxin B1 (AFB1) at an LOD of 0.67 fmol L-1 with high sensitivity and no preamplification. Once the assay is performed, AFB1 is optically identified through an aptamer competition process, which triggers the trans-cleavage activity of Cas14a, resulting in significant color changes on the wood surface upon introducing chromogenic substrates. This method can be conveniently extended to the detection of disease-related protein biomarkers with low detection thresholds.
More Related Videos
09:03Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
Published on: December 23, 2022
07:59Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025