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Related Experiment Video

Updated: Jun 17, 2026

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
14:54

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Published on: February 12, 2022

Next-Generation Hepatocyte Expansion: Bridging Molecular Reprogramming with Multicellular Organoids for Clinical

Yiyang Zhou1, Min Zeng1, Gongbo Fu2

  • 1Department of Hepatobiliary Surgery II, Guangdong Engineering Technology Research Center of Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

Stem Cell Reviews and Reports
|June 16, 2026
PubMed
Summary

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This summary is machine-generated.

Generating functional hepatocytes at scale is crucial for liver disease treatments. This review explores signaling pathways, in vitro expansion, and organoid technologies to advance hepatocyte generation for clinical applications.

Area of Science:

  • Hepatology and regenerative medicine
  • Molecular biology and signaling pathways
  • Bioengineering and tissue engineering

Background:

  • Orthotopic liver transplantation (OLT) is limited by donor scarcity and immune rejection.
  • Bioartificial liver (BAL) systems and hepatocyte transplantation offer alternatives but require scalable functional hepatocyte generation.
  • Current challenges include controlling hepatocyte proliferation and differentiation for clinical use.

Purpose of the Study:

  • To systematically review the molecular mechanisms regulating hepatocyte proliferation and differentiation.
  • To evaluate current and emerging in vitro hepatocyte expansion strategies.
  • To summarize translational progress and future directions in hepatocyte generation and application.

Main Methods:

Keywords:
Bioartificial liver (BAL)Cell-based therapyHepatocyte expansionLiver organoidsLiver regeneration

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Last Updated: Jun 17, 2026

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
14:54

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Published on: February 12, 2022

Generation of Functional Endodermal Hepatic Organoids
08:45

Generation of Functional Endodermal Hepatic Organoids

Published on: May 2, 2025

  • Systematic review of molecular signaling pathways (Wnt/β-catenin, Hippo-YAP, Notch, TGF-β).
  • Critical evaluation of in vitro expansion techniques: genetic modification vs. non-genome editing.
  • Analysis of next-generation methods: defined media, bioreactors, co-cultures, and liver organoids.
  • Review of translational applications in BAL devices, cell transplantation, and toxicology models.

    • Main Results:

    • Key signaling pathways critically balance hepatocyte proliferation and differentiation.
    • Next-generation in vitro strategies, including liver organoids, show promise for generating functional hepatocytes.
    • CRISPR-Cas9 and bioengineering offer potential solutions for current limitations.

    Conclusions:

    • Advancements in understanding signaling pathways and in vitro culture technologies are crucial for scalable functional hepatocyte generation.
    • Liver organoid technology and bioengineering innovations are bridging the gap between in vitro expansion and in vivo relevance.
    • Further preclinical validation is essential for translating these technologies into clinical practice for liver disease treatment.