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Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

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Updated: Jun 20, 2026

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

ROS-Mediated Controllable Targeted Protein Degradation: Progress and Reflections.

Ti Meng1, Hang Zhong1, Yuanyuan Li2

  • 1Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Provincial Key Laboratory of Innovation and Manufacturing for Pharmaceuticals, School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, P. R. China.

Journal of Medicinal Chemistry
|June 19, 2026
PubMed
Summary
This summary is machine-generated.

Controllable targeted protein degradation using reactive oxygen species (ROS) offers precise control for drug discovery. This perspective explores ROS degraders, highlighting their potential and challenges for therapeutic applications.

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Published on: May 15, 2019

Area of Science:

  • Medicinal Chemistry
  • Chemical Biology
  • Drug Discovery

Background:

  • Controllable targeted protein degradation is an innovative strategy in drug discovery.
  • Reactive oxygen species (ROS)-mediated degradation offers spatiotemporal precision, an alternative to conventional Proteolysis-Targeting Chimeras (PROTACs).

Purpose of the Study:

  • To outline the design principles, mechanisms, and advances of ROS-mediated degraders.
  • To critically assess the advantages, barriers, and limitations of ROS degraders for clinical translation.
  • To provide guidance for developing next-generation controllable targeted protein degradation platforms.

Main Methods:

  • Review of photodynamic, photothermal, and sonodynamic ROS degraders.
  • Assessment of druggability, clinical translation barriers, and limitations.
  • Analysis of rational design and therapeutic applications.

Main Results:

  • ROS degraders offer precise control over protein degradation.
  • Photodynamic, photothermal, and sonodynamic approaches represent key advancements.
  • Significant potential exists, but clinical translation faces barriers.

Conclusions:

  • ROS-mediated protein degradation is a promising therapeutic strategy.
  • Further research is needed to overcome limitations and optimize clinical translation.
  • Medicinal chemistry-focused guidance is crucial for future development.