Multifunctional BiomimicCopper Nanozyme as Glucose and Hydrogen Peroxide Biosensor

Area of Science:

• Materials Science
• Electrochemistry
• Nanotechnology

Background:

• Nonenzymatic electrochemical nanozymes are crucial for biomarker sensing.
• Copper-based heteronanostructures offer promising properties for electrochemical applications.

Purpose of the Study:

• To synthesize and characterize Cu/Cu₂O/CuO heteronanostructure-based nanozymes.
• To evaluate their efficacy as nonenzymatic electrochemical sensors for glucose and hydrogen peroxide.
• To demonstrate their potential for noninvasive detection in real samples.

Main Methods:

• One-pot hydrothermal synthesis of Cu/Cu₂O/CuO nanozymes.
• Characterization using X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), and X-ray photoelectron spectroscopy (XPS).
• Electrochemical sensing studies for glucose and hydrogen peroxide detection.

Main Results:

• Successfully synthesized Cu/Cu₂O/CuO heteronanostructures with multiple copper oxidation states.
• Demonstrated enhanced material stability and efficient electron transfer.
• Achieved wide linear detection ranges (10 nM–2.8 mM for glucose, 10 nM–14 mM for H₂O₂) with low detection limits (764 nM for glucose, 87 nM for H₂O₂).
• Validated sensor performance in real samples.

Conclusions:

• Cu/Cu₂O/CuO nanozymes provide a stable and efficient nonenzymatic electrochemical sensing platform.
• The tailored synthesis strategy enables precise control over surface composition and enhances sensing performance.
• This nanozyme holds significant potential for noninvasive glucose and hydrogen peroxide monitoring.