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Luteococcus struthionis sp. nov. and Lacrimispora struthionigena sp. nov. isolated from ostrich faeces
Samuel L Miller1, R Ty Young1, Md Mehedi Hasan1
11 Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK, USA.
Abstract:
Two mesophilic, Gram-stain-positive bacteria, OSA5T and AGF001T, were isolated anoxically from ostrich faecal material. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences revealed that strains OSA5T and AGF001T formed distinct lineages within the genera Luteococcus and Lacrimispora, respectively. Strain OSA5T showed the closest relatedness to Luteococcus japonicus DSM 10546T and Luteococcus sediminum DSMZ 27277T with 96.6% 16S rRNA gene sequence similarity. Strain AGF001T was closely related to Lacrimispora aerotolerans DSM 5434T, with 97.4% 16S rRNA gene sequence similarity. Subsequent genomic analyses using overall genomic-based relatedness indices revealed that OSA5T and AGF001T shared average nucleotide identity and digital DNA-DNA hybridization values <95.0% and <70.0% with their close relatives, below the currently accepted thresholds for species-level delineation. The genomes of strains OSA5T and AGF001T were 3.2 Mbp and 5.4 Mbp, with G+C contents of 67.8 and 41.6 mol%, respectively. Based on phenotypic and genotypic data, strains OSA5T and AGF001T represent novel species in the genera Luteococcus and Lacrimispora, respectively; the names Luteococcus struthionis sp. nov. and Lacrimispora struthionigena sp. nov. are proposed. The type strains are OSA5T (=CCM 9469T=CCUG 78312T=CECT 31229T=DSM 119679T) and AGF001T (=CCM 9472T=CCUG 78314T=CECT 31227T=DSM 119664T), respectively.
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