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Updated: Jun 24, 2026

Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles (PPAs) and Related Biomaterials
Published on: June 25, 2018
Structured odd-chain PUFA TAGs in Pythium: a precursor-guided biosynthesis strategy
Markéta Kulišová1, Irena Jarošová Kolouchová1, Lucie Kyselová2
1Department of Biotechnology, University of Chemistry and Technology, Technická 5, 166 28 Prague, Czech Republic.
Abstract:
Structured triacylglycerols (TAGs) were biosynthetically engineered in Pythium oligandrum ATCC 38472 using a precursor-directed approach. KEGG pathway analysis confirmed that TAG assembly proceeds via the Kennedy pathway. Supplementation with valine, leucine, isoleucine, propionic acid, and pivalic acid promoted the incorporation of odd-chain polyunsaturated fatty acids (odd-PUFAs), with isoleucine, particularly in combination with TWEEN 80, showing the greatest effect. Lipidomic profiling (RP-HPLC-MS/MS) revealed enhanced odd-PUFA esterification at the sn-1 position of lysophosphatidic and phosphatidic acids, while diacylglycerols acted as key intermediates. Two-dimensional and chiral LC-MS/MS confirmed that TWEEN 80 modulates PUFA distribution within TAGs, enabling controlled molecular design. These findings demonstrate a sustainable microbial route for producing structured lipids enriched in odd-PUFAs. The resulting TAGs exhibit tunable structural and nutritional attributes, supporting their potential use as functional lipid ingredients in food formulations and nutraceutical applications.
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