Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
DNA Packaging00:58

DNA Packaging

Overview
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-Spatially Confined Assembly of DNA Nanowall Stiffens Tumor Cells to Enhance Adoptive T-Cell Immunotherapy.

Journal of the American Chemical Society·2026
Same author

Coordination-driven liquid-to-solid phase transition and self-assembly of DNA-metal condensates.

Biomaterials science·2026
Same author

Programmable Circular Single-Stranded DNA Acts as Recyclable Anti-miRNA Nucleotides to Inhibit Colorectal Cancer.

Small methods·2026
Same author

Switchable DNA-Scaffolded Multivalent Aptamer through Stimuli-Responsive Plug-and-Play Modules for Protein Regulation.

ACS applied materials & interfaces·2026
Same author

Advances and challenges in non-canonical nucleic acids data storage.

Nature communications·2026
Same author

Hierarchical access to encoded data on DNA nanostructures using administrator and user keys.

Nucleic acids research·2025
Same journal

Erratum for the Research Article "Assessing the health risks of rice cadmium content standards in China" by H. Chu <i>et al</i>.

Science advances·2026
Same journal

Erratum for the Research Article "Developmental regulation of Erk signaling by mitotic kinases" by F. Chen <i>et al</i>.

Science advances·2026
Same journal

Magnetically levitated metasurface enabling tangible and bidirectional human-machine interaction.

Science advances·2026
Same journal

A general photoinduced manganese-catalyzed platform for the sequential difunctionalization of [1.1.1]propellane.

Science advances·2026
Same journal

Turning sound and force into light with AlN:Mn<sup>2+</sup> mechanoluminescence.

Science advances·2026
Same journal

Extreme dominance of Earth-origin heavy ions in the intense ring current near the Earth during the May 2024 super geomagnetic storm.

Science advances·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Long-stranded XNA-cssDNA hybrids for robust data storage.

Xinyu Sun1,2, Yufeng Pei2, Pan Tan3,4,5

  • 1Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.

Science Advances
|June 24, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel XNA-cssDNA hybrid for robust DNA data storage, enhancing stability and enabling 100% data recovery even after degradation. The new method utilizes FANA polymerase for faster, longer strand synthesis, improving data storage solutions.

More Related Videos

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Related Experiment Videos

Last Updated: Jun 26, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Data Storage

Background:

  • DNA offers high-density, low-energy data storage potential.
  • Long-stranded DNA storage faces stability challenges.
  • Xeno nucleic acids (XNA) present an alternative for enhanced stability.

Purpose of the Study:

  • To develop a robust DNA-based data storage system.
  • To improve the stability and synthesis of DNA for data storage.
  • To achieve high-fidelity data writing and reading.

Main Methods:

  • A long-stranded xeno nucleic acid-circular single-stranded DNA (XNA-cssDNA) hybrid strategy was developed.
  • 2'-fluoro-arabinonucleic acid (FANA) was identified as the optimal XNA.
  • A temperature-guided language model evolved a faster FANA polymerase (Tgomut) for synthesizing long strands (>7500 nt).
  • Data was encoded onto cssDNA using M13 bacteriophage.

Main Results:

  • FANA-cssDNA hybrids demonstrated significant resistance to chemical and enzymatic degradation.
  • The evolved Tgomut polymerase showed a ~4.4-fold increase in speed compared to TgoD4K.
  • A dual-strategy framework achieved 100% data recovery, even after DNA degradation.
  • Encoded digital data and a gene were successfully visualized in mammalian cells.

Conclusions:

  • The FANA-cssDNA hybrid strategy significantly enhances DNA data storage robustness.
  • Engineered polymerases overcome synthesis limitations, enabling longer DNA strands for data encoding.
  • This approach offers a stable and reliable method for DNA data storage and retrieval, with potential for in-cell data applications.