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Microbes in Food Production01:29

Microbes in Food Production

Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
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Extraction of Plant-based Capsules for Microencapsulation Applications
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Sulforaphane Microcapsules via O/W Emulsion: Development, Characterization, and Application in Functional Yogurt.

Yipsy Arozarena1, Víctor Zambrano1, Rubén Bustos1

  • 1Department of Chemical and Bioprocess Engineering, Engineering Faculty, University of Santiago of Chile, Avenida Libertador Bernardo O'Higgins 3363, Central Station, Santiago 9170019, Chile.

Foods (Basel, Switzerland)
|June 26, 2026
PubMed
Summary

Sulforaphane (SFN) stability is enhanced through microencapsulation in gum arabic for yogurt. This method significantly improves SFN retention and preserves its anti-cancer properties in food products.

Keywords:
dairy productsfunctional foodsgum arabicstabilizationsulforaphane

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Area of Science:

  • Food Science
  • Nutritional Biochemistry
  • Materials Science

Background:

  • Sulforaphane (SFN), a potent anti-cancer compound from Brassicaceae vegetables, is unstable and degrades easily.
  • Heat, oxygen, and alkaline conditions compromise SFN's integrity, limiting its application in food products.
  • Microencapsulation offers a promising strategy to protect SFN and enhance its bioavailability.

Purpose of the Study:

  • To develop and optimize microencapsulation of sulforaphane (SFN) using gum arabic (GA) as a wall material.
  • To evaluate the stability and efficacy of SFN microcapsules for incorporation into yogurt.
  • To determine the optimal conditions for SFN microencapsulation via response surface methodology.

Main Methods:

  • Oil-in-water emulsion technique utilizing gum arabic (GA) as the wall material for SFN microencapsulation.
  • Response surface methodology (RSM) to optimize microencapsulation parameters: stirring time, SFN/GA ratio, and surfactant concentration.
  • Characterization of microcapsule morphology, size, and entrapment efficiency; assessment of SFN stability in microcapsules under freezing conditions and in yogurt over time.

Main Results:

  • Optimized microencapsulation achieved 90.0 ± 3.0% entrapment efficiency under conditions of 7 min stirring, 0.7 SFN/GA ratio, and 7% surfactant concentration.
  • Microcapsules exhibited a regular spherical shape (0.5-5.5 µm diameter) with no structural defects.
  • Microencapsulated SFN showed 100% stability at -4 °C for 90 days and 57% retention in yogurt after 30 days, a 14-fold reduction in degradation rate compared to free SFN.

Conclusions:

  • Gum arabic-based microencapsulation effectively stabilizes sulforaphane (SFN), preserving its integrity and anti-cancer potential.
  • The developed microencapsulation technique is suitable for incorporating SFN into yogurt, maintaining product quality.
  • This approach significantly enhances SFN stability and bioavailability in functional food applications.