Genomic Monitoring and Engineering Stable and Safe Immortalized Cell Platforms for Industrial Cellular Agriculture

Area of Science:

• Cell Biology
• Biotechnology
• Food Science

Background:

• Cultivated meat production necessitates advancements in cell line engineering, tissue engineering, and regulatory frameworks.
• Key challenges include developing stable, safe cell platforms and scalable manufacturing processes.

Purpose of the Study:

• To review the cultivated meat developmental pipeline from cell sourcing to regulatory evaluation.
• To compare different cell platforms and immortalization strategies for cultivated meat.
• To identify technical priorities for industrial implementation.

Main Methods:

• Comparative analysis of muscle satellite cells, mesenchymal stromal/adipogenic progenitors, and induced pluripotent stem cells.
• Evaluation of immortalization strategies (senescence bypass, telomerase reactivation, CRISPR).
• Review of advancements in serum-free media, biomaterials, and co-culture protocols.

Main Results:

• Trade-offs exist among cell platforms regarding proliferation, lineage commitment, genomic stability, and food safety.
• Immortalization strategies impact genomic stability and food safety risks.
• Scalable tissue engineering faces bottlenecks in cost, reproducibility, and scale; environmental benefits depend on energy and growth factor optimization.

Conclusions:

• Genomic monitoring is crucial for assessing biological stability, predictability, and suitability for food production.
• Integrating cell biology, biotechnology, and bioprocess engineering is vital for industrializing cultivated meat.
• Clear regulatory frameworks are needed to distinguish food-grade cells from other applications.